Detection of water-borne pathogens: culture plate to genomics
نویسندگان
چکیده
منابع مشابه
Microbial Diagnostic Microarrays for the Detection and Typing of Food- and Water-Borne (Bacterial) Pathogens
Reliable and sensitive pathogen detection in clinical and environmental (including food and water) samples is of greatest importance for public health. Standard microbiological methods have several limitations and improved alternatives are needed. Most important requirements for reliable analysis include: (i) specificity; (ii) sensitivity; (iii) multiplexing potential; (iv) robustness; (v) spee...
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A microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogenetically robust gyrB marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% r...
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The effect of different compost-soil ratios on the incidence of Pythium aphanidermatum causing tomato damping off was tested in a greenhouse experiment. The inoculum density of 5% was determined to be suitable for inoculation in compost experiments based on investigations of disease incidence with 5, 10 and 20% inoculum densities. To evaluate the effect of compost on incidence of tomato damping...
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MassTag polymerase chain reaction (PCR) is a platform that enables microbe detection using primers labeled through a photocleavable link with tags that vary in molecular weight. After multiplex PCR, tags are released by ultraviolet irradiation and analyzed by mass spectroscopy. The identification of a microbe in a sample is determined by its cognate tags. Here we describe establishment and impl...
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ژورنال
عنوان ژورنال: Indian Journal of Science and Technology
سال: 2009
ISSN: 0974-6846,0974-5645
DOI: 10.17485/ijst/2009/v2i11.16